Survivin, a Novel Target of CD44-Promoted Breast Tumor Invasion

Publication year: 2011
Source: The American Journal of Pathology, In Press, Uncorrected Proof, Available online 14 June 2011

Mohamed E., Abdraboh , Rajiv L., Gaur , Andrew D., Hollenbach , Dane, Sandquist , Madhwa H.G., Raj , …

The hyaluronan (HA) receptor CD44 plays an essential role in cell–cell or cell–extracellular matrix communications and is a bioactive signal transmitter. Although a number of studies have described the function of CD44 in breast cancer (BC) metastasis, the underlying mechanisms have yet to be determined. By using a validated tetracycline-off-regulated CD44 expression system in the MCF-7 cell line combined with microarray analysis, we identified survivin (SVV) as a potential downstream transcriptional target of CD44. To test the hypothesis that SVV underpins CD44-promoted BC cell invasion, we combined molecular and pharmacologic approaches and showed that CD44 induction increased SVV expression levels,…