Safety evaluation of steroidal saponin DT-13 isolated from the tuber of Liriope muscari (Decne.) Baily

Publication year: 2011
Source: Food and Chemical Toxicology, In Press, Accepted Manuscript, Available online 13 June 2011

Shengtang, Ma , Junping, Kou , Boyang, Yu

Steroidal saponin DT-13 (25 (R, S)-ruscogenin-1-O-[β-D- glucopyranosyl – (1→2)] [β-D-xylopyranosyl-(1→3)]-β-D-fucopyranoside) is the main active component of the tube of Liriope muscari (Decne.) Baily and has been studied as a candidate drug for cancer metastasis. The objective of this study was to evaluate the safety of DT-13 systematically by genotoxicity and acute oral toxicity and subchronic 90-day oral gavage toxicity. Results of Ames test confirmed that DT-13 did not induce mutations in histidine auxotrophs Salmonella typhimurium (TA 97, TA98, TA100, and TA102) both in the presence and absence of metabolic activation system at the doses of 0.05-500 μg/plate. Meanwhile, DT-13 did…

 Highlights: ► Steroidal saponin DT-13 (25 (R, S)-ruscogenin-1-O-[β-D- glucopyranosyl – (1→2)] [β-D-xylopyranosyl-(1→3)]-β-D-fucopyranoside) from the tube of Liriope muscari (Decn.) Bailey shows no genotoxicity in Ames test and mouse micronucleus test. ► DT-13 shows low oral acute toxicity with the minimum lethal dose (MLD) more than 5000 mg/kg in mice. ► DT-13 shows low oral subchronic toxicity at the doses of 10, 60 and 360 mg/kg in rats. ► DT-13 shows lighter sensitivity to female mice.