Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas

Publication year: 2011
Source: Ecotoxicology and Environmental Safety, In Press, Corrected Proof, Available online 14 June 2011

Agnieszka, Babczyńska , Grażyna, Wilczek , Elżbieta, Szulińska , Izabella, Franiel

Spiders inhabiting post industrial environments, such as waste heaps or ore-bearing areas, are exposed to high concentrations of metals, accumulated in the body of their prey and transferred along food chains. Therefore spiders are pressed to develop metal-neutralization strategies. Low-molecular, multifunction proteins: metallothioneins (MTs), often postulated as biomarkers of metal exposure, are known to bind metals and thus protect organisms against their toxic effects. Yet the proteins are still not well recognized in spiders. The aim of this study was to assess, by immunodetection method, ELISA, the concentration of metallothioneins in adult females of three web building spider species: Araneus…

Graphical abstract

 Graphical abstract:  Highlights: ► ELISA was used for metallothionein immunodetection in spiders. ► Female of three spider species were collected from variously polluted sites. ► Metallothioneins concentration in spiders reflects the level of metals in their habitat. ► Metallothioneins are important in metal neutralization or regulation in spiders. ► Metallothioneins level is species specific.