Pancreatic Exocrine Enzyme-producing Cell Differentiation via Embryoid Bodies from Human Embryonic Stem Cells

Publication year: 2011
Source: Biochemical and Biophysical Research Communications, In Press, Accepted Manuscript, Available online 13 June 2011

Sakiko, Shirasawa , Susumu, Yoshie , Fengming, Yue , Hinako, Ichikawa , Tadayuki, Yokoyama , …

Mouse embryonic stem cells (ESCs) can be induced to form pancreatic exocrine enzyme-producing cells in vitro in a stepwise fashion that recapitulates the development in vivo. However, there is no protocol for the differentiation of pancreatic-like cells from human ESCs (hESCs). Based upon the mouse ESC model, we have induced the in vitro formation of pancreatic exocrine enzyme-producing cells from hESCs. The protocol took place in four stages. In Stage 1, embryoid bodies (EBs) were formed from dissociated hESCs and then treated with the growth factor activin A, which promoted the expression of Foxa2 and Sox17 mRNAs, markers of definitive…

 Highlights: ► There is no protocol for the differentiation of pancreatic-like cells from human ESCs (hESCs). ► We have induced pancreatic exocrine enzyme-producing cells from hESCs based upon our previous mouse ESC model. ► FGF7 play the important part in inducing hESCs into exocrine-like cells. ► At the end of this procedure, the differentiated cells were demonstrated to express pancreas-specific enzymes, such as amylase, elastase, carboxypeptidase A and chymotrypsin.