Optimized Ferrocene-Functionalized ZnO Nanorods for Signal Amplification in Electrochemical Immunoassay of Escherichia coli

Publication year: 2011
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 13 June 2011

Yingqiao, Teng , Xinai, Zhang , Ying, Fu , Huijie, Liu , Zhongchuan, Wang , …

A novel amplified electrochemical immunoassay based on ferrocene (Fc)-functionalized ZnO nanorods (NRs) was developed in the present work. The detection antibody (dAb) and Fc were immobilized onto the surface of ZnO NRs, denoted as {dAb-ZnO-Fc} bioconjugates. The amount of dAb and Fc in the bioconjugates was investigated using the copper reduction/bicinchoninic acid reaction (BCA protein assay) and inductive coupled plasma-atomic emission spectroscopy (ICP-AES), respectively. Greatly amplified signal was achieved in the sandwich-type immunoassay when dAb and Fc linked to ZnO NRs at a proper ratio. Using Escherichia coli (E. coli) as a model antigen, the designed immunoassay showed an excellent…