Influence of over-expression of cytosolic aspartate aminotransferase on amino acid metabolism and defence responses against Botrytis cinerea infection in Arabidopsis thaliana

Publication year: 2011
Source: Journal of Plant Physiology, In Press, Corrected Proof, Available online 14 June 2011

Sigrid, Brauc , Eline, De Vooght , Martine, Claeys , Monica, Höfte , Geert, Angenon

Arabidopsis possesses several genes encoding aspartate aminotransferase, which catalyzes the bidirectional conversion of aspartate into glutamate. These amino acids together with asparagine and glutamine play an important role in N storage and distribution. In addition, they act as precursors for other amino acids. The gene encoding cytosolic aspartate aminotransferase, Asp2, was found to be induced upon infection with the necrotrophic pathogen Botrytis cinerea in Arabidopsis. Asp2 over-expression lines and a T-DNA insertion mutant were used to study the role of aspartate aminotransferase in Arabidopsis defence responses. Over-expression of Asp2 led to changes in aspartate content and aspartate-derived amino acids. The…