Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range

Publication year: 2011
Source: Marine Genomics, In Press, Corrected Proof, Available online 14 June 2011

Sónia I., Massa , Gareth A., Pearson , Tânia, Aires , Michael, Kube , Jeanine L., Olsen , …

Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera noltii during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30min, 2h, 4h and 24h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as…

 Highlights: ► Response to heat stress is very fast but gene expression returns to normal after 24h. ► Photosynthesis-related genes were under-expressed after heat-shock. ► Heat-shock caused a quick rise in heat shock proteins and molecular chaperone expression.