Efficient gene targeting in ΔCc.ku70 or ΔCc.lig4 mutants of the agaricomycete Coprinopsis cinerea

Publication year: 2011
Source: Fungal Genetics and Biology, In Press, Accepted Manuscript, Available online 13 June 2011

Takehito, Nakazawa , Yuki, Ando , Kohei, Kitaaki , Kiyoshi, Nakahori , Takashi, Kamada

Coprinopsis cinerea is a model for studies of sexual development in agaricomycetes (homobasidiomycetes). Efficient gene targeting should facilitate such studies, especially because increasing genome and transcriptome information is now available in C. cinerea. To estimate the frequency of gene disruption by homologous integration in this fungus, we tried to disrupt Cc.wc-2, which encodes a WC-2 homolog, a partner of the fungal blue-light photoreceptor, WC-1. Disruption of Cc.wc-2 did not occur when recipients (protoplasts) of the disrupting construct were prepared from asexual spores, oidia, from the wild type, 326, while it occurred when protoplasts were prepared from mycelial cells from the…

 Highlights: ► We disrupted ku70 and lig4 homologs in a model mushroom, Coprinopsis cinerea. The disruption of Cc.ku70 or Cc.lig4 greatly enhanced gene targeting. We verified by gene targeting that Cc.wc-2 is involved in perception of blue light.