Effects of cadmium on proliferation and self-renewal activity of prostate stem/progenitor cells

Publication year: 2011
Source: Environmental Toxicology and Pharmacology, In Press, Accepted Manuscript, Available online 13 June 2011

Gaofeng, Jiang , Lei, Xu , Bo, Zhang , Lei, Wu

Cadmium (Cd) is an occupational and environmental pollutant that induces numerous pathological effects, including injuries to prostate. The aim of the present study was to investigate the effects of Cd on self-renewal and proliferation of prostate stem/progenitor cells (PSPC) and its possible mechanisms. Prostate epithelial cells were prepared from mice to form sphere in Matrigel/PrEGM supplemented with cadmium chloride (CdCl2). The data showed that CdCl2 inhibits sphere-forming ability and proliferation of PSPC in a concentration dependent manner. Primary spheres were then passaged to form daughter spheres and we found that CdCl2 suppressed PSPC self-renewal activity, which recovered after further passaging….