Differential contribution of XPC, RAD23A, RAD23B and CENTRIN 2 to the UV-response in human cells

Publication year: 2011
Source: DNA Repair, In Press, Corrected Proof, Available online 14 June 2011

Emilie, Renaud , Laurent, Miccoli , Natalie, Zacal , Denis S., Biard , Constantin T., Craescu , …

Several genes in human cells are activated by physical genotoxic agents in order to regenerate cell homeostasis. Among the pathways contributing to this response, nucleotide excision repair (NER) is unique in restoring the nucleotide sequence of the DNA molecule without generating mutations. The first step of NER is mediated by a protein complex composed of XPC, RAD23B, an ubiquitin receptor and CENTRIN 2, an EF-hand calcium binding protein. These three proteins are multifunctional and participate in other important biochemical pathways. We silenced the XPC, RAD23A or RAD23B genes in HeLa cells for a long period of time by using Epstein…

 Highlights: ► We characterized the first steps of global-genome repair in human cells. ► We examined the UVC-response of cells silenced for XPC and RAD23Aand/orB genes. ► RAD23A and RAD23B display distinct roles in DNA repair. ► XPC is essential for the localization of CENTRIN 2 to nuclear damaged areas. ► The CENTRIN 2 trapping may indirectly perturb proliferation and contribute to NER.