Contribution of laboratory methods in diagnosing clinically suspected ocular toxoplasmosis in Brazilian patients☆

Publication year: 2011
Source: Diagnostic Microbiology and Infectious Disease, Volume 70, Issue 3, July 2011, Pages 362-366

Cinara C.B., Mattos , Cristina S., Meira , Ana I.C., Ferreira , Fábio B., Frederico , Roberto M., Hiramoto , …

This prospective study evaluated the value of laboratorial diagnosis in ocular toxoplasmosis analyzing peripheral blood samples from a group of Brazilian patients by immunologic and molecular methods. We analyzed blood samples from 184 immunocompetent patients with ocular disorders divided into 2 groups: Group I, composed of samples from 49 patients with ocular toxoplasmosis diagnosed by clinical features; Group II, samples from 135 patients with other ocular diseases. Samples were assayed by conventional polymerase chain reaction (cnPCR), real-time PCR (qPCR) for Toxoplasma gondii, indirect immunofluorescence reaction (IF), avidity test (crude tachyzoite lysate as antigen), and excreted–secreted tachyzoite proteins as antigen (ESA-ELISA)….