Cadmium-inducible BgMT2, a type 2 metallothionein gene from mangrove species (Bruguiera gymnorrhiza), its encoding protein shows metal-binding ability

Publication year: 2011
Source: Journal of Experimental Marine Biology and Ecology, In Press, Corrected Proof, Available online 14 June 2011

Guo-Yong, Huang , You-Shao, Wang , Guang-Guo, Ying

Metallothioneins (MTs) are a family of low-molecular-weight Cys-rich proteins and are thought to play possible role in metal metabolism or detoxification. To shed light on the role of type 2 MT in Bruguiera gymnorrhiza (BgMT2) under heavy metal stress, B. gymnorrhiza seedlings were exposed to different concentrations of CdCl2 (2μM, 10μM, 20μM or 40μM) for 3, 7 and 11d. Real-time quantitative PCR analysis demonstrated that BgMT2 gene transcripts in leaves of B. gymnorrhiza increased in all Cd concentrations and exposure durations. BgMT2 was overexpressed in Escherichia coli BL21 (DE3) as a fusion protein (GST-BgMT2), and bacteria expressing the fusion protein…

 Research highlights: ► A real-time quantitative PCR protocol was developed to directly evaluate mangrove metallothionein gene expression. ► Cadmium could induce mangrove metallothionein gene expression ► Mangrove metallothionein gene was overexpressing in Escherichia coli. ► Mangrove metallothionein could enhance E. coli tolerance to heavy metals by chelating them.