2’,5’-Dihydroxychalcone-induced glutathione is mediated by oxidative stress and kinase signaling pathways

Publication year: 2011
Source: Free Radical Biology and Medicine, In Press, Accepted Manuscript, Available online 13 June 2011

Remy, Kachadourian , Subbiah, Pugazhenthi , Kalpana, Velmurugan , Donald S., Backos , Christopher C., Franklin , …

Hydroxychalcones are naturally occurring compounds that continue to attract considerable interest due to their anti-inflammatory and anti-angiogenic properties. They have been reported to inhibit the synthesis of the inducible nitric oxide (NO) synthase and to induce the expression of heme oxygenase-1 (HO-1). This study examines the mechanisms by which 2’,5’-dihydroxychalcone (2’,5’-DHC) induces an increase in cellular glutathione (GSH) levels using a cell line stably expressing a luciferase reporter gene driven by antioxidant response elements (MCF-7/AREc32). 2’,5’-DHC-induced increase in cellular GSH levels was partially inhibited by the catalytic antioxidant MnTDE-1,3-IP5+, suggesting that reactive oxygen species (ROS) mediate the antioxidant adaptive response….